preparing poly (lactic-co-glycolic acid) (plga) microspheres containing lysozyme-zinc precipitate using a modified double emulsion method

Authors

nastaran nafissi varcheh department of pharmaceutical biotechnology, school of pharmacy, shaheed beheshti university of medical sciences, tehran, iran.

vera luginbuehl institute of pharmaceutical sciences, eth zurich, zurich, switzerland.

reza aboofazeli department of pharmaceutics, school of pharmacy, shaheed beheshti university of medical sciences, tehran, iran.

hans peter merkle institute of pharmaceutical sciences, eth zurich, zurich, switzerland.

abstract

lysozyme, as a model protein, was precipitated through the formation of protein-zn complex to micronize for subsequent encapsulation within poly (lactic-co-glycolic acid) (plga) microspheres. various parameters, including ph, type and concentration of added salts and protein concentration, were modified to optimize the yield of protein complexation and precipitation. the resulting protein particles (lysozyme-zn complex as a freshly prepared suspension or a freeze-dried solid) were then loaded into plga (resomer® 503h) microspheres, using a double emulsion technique and microspheres encapsulation efficiency and their sizes were determined. it was observed that salt type could significantly influence the magnitude of protein complexation. at the same conditions, zinc chloride was found to be more successful in producing pelletizable lysozyme. generally, higher concentrations of protein solution led also to the higher yields of complexation and at the optimum conditions, the percentage of pelletizable lysozyme reached to 80%. taking advantage of this procedure, a modified technique for preparation of protein-loaded plga microspheres was established, although it is also expected that this technique increases the protein drugs stabilization during the encapsulation process.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Preparing Poly (Lactic-co-Glycolic Acid) (PLGA) Microspheres Containing Lysozyme-Zinc Precipitate Using a Modified Double Emulsion Method

Lysozyme, as a model protein, was precipitated through the formation of protein-Zn complex to micronize for subsequent encapsulation within poly (lactic-co-glycolic acid) (PLGA) microspheres. Various parameters, including pH, type and concentration of added salts and protein concentration, were modified to optimize the yield of protein complexation and precipitation. The resulting protein parti...

full text

Preparing Poly (Lactic-co-Glycolic Acid) (PLGA) Microspheres Containing Lysozyme-Zinc Precipitate Using a Modified Double Emulsion Method

Lysozyme, as a model protein, was precipitated through the formation of protein-Zn complex to micronize for subsequent encapsulation within poly (lactic-co-glycolic acid) (PLGA) microspheres. Various parameters, including pH, type and concentration of added salts and protein concentration, were modified to optimize the yield of protein complexation and precipitation. The resulting protein parti...

full text

Preparing Poly (Lactic-co-Glycolic Acid) (PLGA) Microspheres Containing Lysozyme-Zinc Precipitate Using a Modified Double Emulsion Method

Lysozyme, as a model protein, was precipitated through the formation of protein-Zn complex to micronize for subsequent encapsulation within poly (lactic-co-glycolic acid) (PLGA) microspheres. Various parameters, including pH, type and concentration of added salts and protein concentration, were modified to optimize the yield of protein complexation and precipitation. The resulting protein parti...

full text

Poly (Lactic-Co-Glycolic) Acid (PLGA) Adjuvant for Immunotherapy

Nowadays, there are several efforts in immunotherapy especially biodegradable and biocompatible nano-polymers are using as immunomodulatory to induce cell mediated immune responses [1]. The advantages of nanoparticles have been proved to be potent immunomodulators in several kinds of vaccines to control a release of antigen, higher quality and quantity of immune responses and protect the integr...

full text

Self-encapsulating Poly(lactic-co-glycolic acid) (PLGA) Microspheres for Intranasal Vaccine Delivery.

Herein we describe a formulation of self-encapsulating poly(lactic-co-glycolic acid) (PLGA) microspheres for vaccine delivery. Self-healing encapsulation is a novel encapsulation method developed by our group that enables the aqueous loading of large molecules into premade PLGA microspheres. Calcium phosphate (CaHPO4) adjuvant gel was incorporated into the microspheres as a protein-trapping age...

full text

A simple and robust method for pre-wetting poly (lactic-co-glycolic) acid microspheres

Poly (lactic-co-glycolic) acid microspheres are amenable to a number of biomedical procedures that support delivery of cells, drugs, peptides or genes. Hydrophilisation or wetting of poly (lactic-co-glycolic) acid are an important pre-requisites for attachment of cells and can be achieved via exposure to plasma oxygen or nitrogen, surface hydrolysis with NaOH or chloric acid, immersion in ethan...

full text

My Resources

Save resource for easier access later


Journal title:
iranian journal of pharmaceutical research

جلد ۲۰۱۱، شماره ۶، صفحات ۲۰۳-۲۰۹

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023